flex-ChrimsonR-EYFP-Kv
(Plasmid #135319)

• Purpose: Cre-dependent expression of soma-targeted ChrimsonR
• Depositing Lab: Hongkui Zeng
• Publication: Distribution and strength of interlaminar synaptic connectivity in mouse primary visual cortex revealed by two-photon optogenetic stimulation (unpublished)

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4827
• Total vector size (bp): 6807
• Modifications to backbone: EF1a promoter exchanged for synapsin
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ChrimsonR
• Species: Synthetic
• Insert Size (bp): 1980
• GenBank ID: KF992060
• Promoter: synapsin
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: cttTATACGAAGTTatgCTAGcgccaCCatggctgagctgatca
• 3′ sequencing primer: ATTATACGAAGTTATGGCGCGCCttaaAACCGCGTAGCCTCTGGAA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ChrimsonR sequence amplified from Addgene plasmid #99231

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry