pCDF11-SFPQ(369-598)
(Plasmid #135438)

• Purpose: Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag
• Depositing Lab: Charles Bond
• Publication: Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):3826-40. doi: 10.1093/nar/gkv156. Epub 2015 Mar 12.

Backbone

• Vector backbone: pCDF11
• Backbone manufacturer: EMBL
• Backbone size w/o insert (bp): 3584
• Total vector size (bp): 4275
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: splicing factor proline and glutamine rich
• Species: H. sapiens (human)
• Insert Size (bp): 700
• GenBank ID: NM_005066.3 6421
• Entrez Gene: SFPQ (a.k.a. POMP100, PPP1R140, PSF)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistidine (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CTT TAT TTT CAg ggC gCC CAT gCT gCT gCC CTT TCT g
• 3′ sequencing primer: CCgCTCgAgTTAgCTgTAACT TTCCTC

Resource Information

• Supplemental Documents:
  • pCDF11-SFPQ(369–598).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry