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pcDNA3.1 FLIPE-1m
(Plasmid #13544)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13544 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIP-E A207W
  • Alt name
    ECFP-ybeJ-Venus
  • Alt name
    Glutamate/aspartate binding protein
  • Alt name
    gltI
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    A207W in binding protein
  • GenBank ID
    NP_415188
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Venus is from Dr. Atsushi Miyawaki. Please ask Dr. Miyawaki for permission to use Venus in another vector.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 FLIPE-1m was a gift from Wolf Frommer (Addgene plasmid # 13544 ; http://n2t.net/addgene:13544 ; RRID:Addgene_13544)
  • For your References section:

    Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Okumoto S, Looger LL, Micheva KD, Reimer RJ, Smith SJ, Frommer WB. Proc Natl Acad Sci U S A. 2005 Jun 14. 102(24):8740-5. 10.1073/pnas.0503274102 PubMed 15939876