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pBS L30 Ruby3-Rab7A
(Plasmid #135651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135651 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 135651-D50 50 μg of DNA in Tris buffer $495
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DNA 135651-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBS
  • Modifications to backbone
    Modified for mammalian expression using the promoter of the mouse ribosomal protein L30 gene ("weak promoter") and a HSV TK polyA tail
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab7A
  • Alt name
    Rab7
  • Alt name
    ras-related protein Rab-7a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    624
  • GenBank ID
    NP_004628.4 7879
  • Entrez Gene
    RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
  • Promoter L30
  • Tag / Fusion Protein
    • Ruby3 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS L30 Ruby3-Rab7A was a gift from Raphael Gaudin (Addgene plasmid # 135651 ; http://n2t.net/addgene:135651 ; RRID:Addgene_135651)

  • For your References section:

    Rab7-harboring vesicles are carriers of the transferrin receptor through the biosynthetic secretory pathway. Deffieu MS, Cesonyte I, Delalande F, Boncompain G, Dorobantu C, Song E, Lucansky V, Hirschler A, Cianferani S, Perez F, Carapito C, Gaudin R. Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7803. doi: 10.1126/sciadv.aba7803. Print 2021 Jan. 10.1126/sciadv.aba7803 PubMed 33523982
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