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Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
(Plasmid #135766)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135766 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wilms Tumor 1b gene (N-terminal domain)
  • Alt name
    Wt1b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    792
  • Entrez Gene
    wt1b (a.k.a. si:ch211-160j6.3, wt1, zgc:152697)
  • Promoter UAS

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP was a gift from Nadia Mercader Huber (Addgene plasmid # 135766 ; http://n2t.net/addgene:135766 ; RRID:Addgene_135766)
  • For your References section:

    Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish. Sanz-Morejon A, Garcia-Redondo AB, Reuter H, Marques IJ, Bates T, Galardi-Castilla M, Grosse A, Manig S, Langa X, Ernst A, Piragyte I, Botos MA, Gonzalez-Rosa JM, Ruiz-Ortega M, Briones AM, Salaices M, Englert C, Mercader N. Cell Rep. 2019 Jul 30;28(5):1296-1306.e6. doi: 10.1016/j.celrep.2019.06.091. 10.1016/j.celrep.2019.06.091 PubMed 31365871