integrin beta6 777T pcDNA1 neo
(Plasmid #13582)

• Depositing Lab: Dean Sheppard
• Publication: Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Backbone

• Vector backbone: pcDNAI neo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Plasmid contains SupF. Grow in TOP10/P3 cells.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: integrin beta 6 777T
• Alt name: ITGB6
• Alt name: integrin beta 6
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Mutation: 777T. Lacks last 11 amino acids of cytoplasmic domain.
• GenBank ID: NM_000888
• Entrez Gene: ITGB6 (a.k.a. AI1H)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry