PurposeExpression of Leishmania donovani ectodomain in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||135848||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6132
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameHsp70 protein, putative
Insert Size (bp)2100
/ Fusion Protein
- biotinylation sequence and His tag (C terminal on insert)
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid contains a partial truncation of the AmpR promoter that does not seem to impair its ability to provide functional selection.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:D32_Ld_354780 was a gift from Gavin Wright (Addgene plasmid # 135848 ; http://n2t.net/addgene:135848 ; RRID:Addgene_135848)