D36_Ld_323600
(Plasmid #135850)

• Purpose: Expression of Leishmania donovani ectodomain in mammalian cells
• Depositing Lab: Gavin Wright
• Publication: Leishmania ectodomain expression plasmids (unpublished)

Backbone

• Vector backbone: pTT3
  (Search Vector Database)
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6132
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hypothetical protein, conserved
• Alt name: LDPBK_323600
• Species: Leishmania donovani
• Insert Size (bp): 1602
• Tag / Fusion Protein:
  • biotinylation sequence and His tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry