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(Plasmid #135927)


Item Catalog # Description Quantity Price (USD)
Plasmid 135927 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pAF137 SalI + NheI digest
  • Backbone manufacturer
    Andrew Flies Wild Immunology Laboratory
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    TNFRSF9 fused to mNeptune2
  • Alt name
  • Alt name
  • Species
    Tasmanian devil (Sarcophilus harrisii)
  • Promoter EF1a
  • Tag / Fusion Protein
    • mNeptune2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcctcagacagtggttcaaag
  • 3′ sequencing primer aggcacagtcgaggctgat
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pSBtet_Hyg_60508; pCMV(CAT)T7-SB100_34879; mNeptune2-N1_54837
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See bioRxiv preprint: Please see the following article for protocols on how to construct or use this vector: Flies, A. S., Darby, J. M., Murphy, P. R., Pinfold, T. L., Patchett, A. L. & Lennard, P. R. Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins. Bio-protocol under review (2020)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAF163 was a gift from Andrew S. Flies (Addgene plasmid # 135927 ; ; RRID:Addgene_135927)
  • For your References section:

    A novel system to map protein interactions reveals evolutionarily conserved immune evasion pathways on transmissible cancers. Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De Luca A, Lyons AB, Woods GM, Patchett AL. Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 10.1126/sciadv.aba5031. Print 2020 Jul. 10.1126/sciadv.aba5031 PubMed 32937435