integrin beta6 Y762F, Y774F pBabe puro
(Plasmid #13598)

• Depositing Lab: Dean Sheppard
• Publication: Other integrin beta6 plasmids (unpublished)

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Available at Addgene (plasmid #1764)
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integrin beta 6 Y762F Y774F
• Alt name: ITGB6
• Alt name: integrin beta 6
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Mutation: Y762F Y774F
• GenBank ID: NM_000888
• Entrez Gene: ITGB6 (a.k.a. AI1H)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI/SnaBI (unknown if destroyed)
• 3′ cloning site: XbaI/SnaBI (unknown if destroyed)
• 5′ sequencing primer: pBabe 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for additional restriction enzyme sites.