[pSK600] pRK5 HA-Nprl2(R78A)
(Plasmid
#136146)
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Purposemammalian expression of Nprl2
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNprl2
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SpeciesH. sapiens (human)
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MutationR78A
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Entrez GeneNPRL2 (a.k.a. FFEVF2, NPR2, NPR2L, TUSC4)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
[pSK600] pRK5 HA-Nprl2(R78A) was a gift from David Sabatini & Kuang Shen (Addgene plasmid # 136146 ; http://n2t.net/addgene:136146 ; RRID:Addgene_136146) -
For your References section:
Arg-78 of Nprl2 catalyzes GATOR1-stimulated GTP hydrolysis by the Rag GTPases. Shen K, Valenstein ML, Gu X, Sabatini DM. J Biol Chem. 2019 Feb 22;294(8):2970-2975. doi: 10.1074/jbc.AC119.007382. Epub 2019 Jan 16. 10.1074/jbc.AC119.007382 PubMed 30651352
