Tol2 8xSTAR-mNeonGreen. PGK-puro
(Plasmid
#136255)
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PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
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Depositing Lab
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Depositing OrganizationUniversity Medical Centre Utrecht
Located in Netherlands -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136255 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 136255-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 136255-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepminiTol2, Addgene #31829
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Backbone manufacturerStephen Ekker lab
- Backbone size w/o insert (bp) 3498
- Total vector size (bp) 6360
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Vector typeMammalian Expression ; Tol2 transposase
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namestem cell Ascl2 reporter, 8 repeats
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Alt name8x STAR
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)540
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Tag
/ Fusion Protein
- mNeonGreen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 136255-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 136255-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tol2 8xSTAR-mNeonGreen. PGK-puro was a gift from Hugo Snippert (Addgene plasmid # 136255 ; http://n2t.net/addgene:136255 ; RRID:Addgene_136255) -
For your References section:
Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive Minigene. Oost KC, van Voorthuijsen L, Fumagalli A, Lindeboom RGH, Sprangers J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG. Cell Rep. 2018 Feb 6;22(6):1600-1614. doi: 10.1016/j.celrep.2018.01.033. 10.1016/j.celrep.2018.01.033 PubMed 29425513
Map uploaded by the depositor.
