pAEF6
(Plasmid
#136303)
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PurposepAEF5 plasmid with two gRNA inserted
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGangZhao110
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Backbone manufacturerBruce Futcher laboratory at Stony Brooks University
- Backbone size w/o insert (bp) 10864
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Modifications to backboneleu2 gene on the pGangZhao110 is replaced by hph gene
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Vector typeCRISPR ; template for PCR to insert double gRNA coding sequence in pAEF5
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedouble cassette coding for gRNA
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SpeciesSynthetic
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Insert Size (bp)410
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAEF6 was a gift from Gilles Fischer (Addgene plasmid # 136303 ; http://n2t.net/addgene:136303 ; RRID:Addgene_136303) -
For your References section:
A Versatile Protocol to Generate Translocations in Yeast Genomes Using CRISPR/Cas9. Agier N, Fleiss A, Delmas S, Fischer G. Methods Mol Biol. 2021;2196:181-198. doi: 10.1007/978-1-0716-0868-5_14. 10.1007/978-1-0716-0868-5_14 PubMed 32889721
Map uploaded by the depositor.
