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(Plasmid #136435)


Item Catalog # Description Quantity Price (USD)
Plasmid 136435 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 13705
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    platelet-derived growth factor receptor alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Y206S (disrupts PDGF binding, but still binds HCMV trimer)
  • GenBank ID
  • Entrez Gene
    PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Signal peptide from influenza HA (N terminal on insert)
    • Extracellular c-myc epitope tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-myc-PDGFRalpha-Y206S was a gift from Erik Procko (Addgene plasmid # 136435 ; ; RRID:Addgene_136435)
  • For your References section:

    Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E. PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. 10.1371/journal.ppat.1008647 PubMed 32559251