OSCA3.1
(Plasmid
#136596)
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PurposeExpresses the mechanosensitive channel OSCA3.1 in mammalian cells
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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 136596-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 136596-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRES2-mCherry
- Backbone size w/o insert (bp) 5286
- Total vector size (bp) 7460
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSCA3.1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2175
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GenBank IDNM_102773.3
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Entrez GeneERD4 (a.k.a. AT1G30360, T4K22.4, T4K22_4, early-responsive to dehydration 4)
- Promoter CMV
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Tag
/ Fusion Protein
- pIRES2-mCherry (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer IRES Reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySequence was downloaded from TAIR [The arabidopsis information resource] and human codon-optimized and synthesized by Genewiz.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 136596-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 136596-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OSCA3.1 was a gift from Ardem Patapoutian (Addgene plasmid # 136596 ; http://n2t.net/addgene:136596 ; RRID:Addgene_136596) -
For your References section:
OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels. Murthy SE, Dubin AE, Whitwam T, Jojoa-Cruz S, Cahalan SM, Mousavi SAR, Ward AB, Patapoutian A. Elife. 2018 Nov 1;7. pii: 41844. doi: 10.7554/eLife.41844. 10.7554/eLife.41844 PubMed 30382938
Map uploaded by the depositor.
