PurposeAcceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||136698||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
MutationBsaI/ SapI domesticated
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCk4 was a gift from Nicola Patron (Addgene plasmid # 136698 ; http://n2t.net/addgene:136698 ; RRID:Addgene_136698)
For your References section:Systematic tools for reprogramming plant gene expression in a simple model, Marchantia polymorpha. Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, Markel K, Delmans M, West A, Patron NJ, Haseloff J. ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511. 10.1021/acssynbio.9b00511 PubMed 32163700