pISH-NQO1
(Plasmid
#136766)
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PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Nqo1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM-T-Easy
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Vector typein situ probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNqo1
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SpeciesM. musculus (mouse)
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MutationISH Probe insert, V184A- Please see depositor comments below.
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Entrez GeneNqo1 (a.k.a. AV001255, Dia4, Dtd, Nmo-1, Nmo1, Nmor1, Ox-1, Ox1, Qr1)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ISH probe for mouse gene: NQO1
Depositor confirms V184A mutation in NQO1 does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-NQO1 was a gift from Peter Mombaerts (Addgene plasmid # 136766 ; http://n2t.net/addgene:136766 ; RRID:Addgene_136766) -
For your References section:
The Zonal Organization of Odorant Receptor Gene Choice in the Main Olfactory Epithelium of the Mouse. Zapiec B, Mombaerts P. Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 10.1016/j.celrep.2020.02.110. 10.1016/j.celrep.2020.02.110 PubMed 32209480
