pJBL7010
(Plasmid #136942)

• Purpose: Constitutive sfGFP expression (high), bacterial promoter
• Depositing Lab: Julius Lucks
• Publication: Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414. doi: 10.1021/acssynbio.8b00430. Epub 2019 Jan 29.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 3122
• Total vector size (bp): 3906
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sfGFP
• Species: Synthetic
• Insert Size (bp): 784
• Promoter: J23119

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTTTTCCCAGTCACGAC
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Supplemental Documents:
  • pADS7010.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cells harboring this plasmid are neon-green on a plate but should only be used for cell-free expression--seems to be toxic for cellular expression to some extent.