pGGA_RT_Sa_Cas9
(Plasmid
#136962)
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PurposeGoldengate/Greengate module for genome editing, using the SaCAS9.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSaCAS9
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate promoter module containing the SaCAS9 driven by the parsley Ubiquitin promoter for genome editing.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGA_RT_Sa_Cas9 was a gift from Klaus Theres (Addgene plasmid # 136962 ; http://n2t.net/addgene:136962 ; RRID:Addgene_136962)
