pB6-Gfap-T2A-td-sfGFP-FNF-DTA
(Plasmid #137044)

• Purpose: B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: pDTA-5
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Gfap
• Species: M. musculus (mouse)
• Entrez Gene: Gfap
• Tag / Fusion Protein:
  • T2A-td-sfGFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pGfap-T2A-tdGFP-FNF-DTA5.gb
• A portion of this plasmid was derived from a plasmid made by: sfGFP is from Sandia Biotech. FRT-Neo-FRT is from pFNF (#22687).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A targeting vector to knock-in T2A-td-sfGFP into mouse Gfap protein isoform 2 (also known as Gfap-alpha). This is the most abundant isoform in the mouse brain. Of note, the Gfap protein transcript variant 2 transcript level is high in the SVZ, an adult neural stem cell niche. See Kamphuis et al., 2012 (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042823).

Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.