pCpSRb-4
(Plasmid #13726)

• Depositing Lab: Thomas Schwartz
• Publication: Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 103(18):6823-8.

Backbone

• Vector backbone: pET28a-derived
• Backbone size w/o insert (bp): 5312
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Expression is optimal at 30 degrees
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Signal recognition particle receptor β subunit
• Alt name: SRβ
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 576
• Mutation: Circularly permuted SRβD210K183 with Precission protease cleavable 6x-His tag
• Entrez Gene: SRP102
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry