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Pprm1-luciferase; his-3
(Plasmid #137728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137728 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 137728-D50 50 μg of DNA in Tris buffer $495
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DNA 137728-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pMF272
  • Vector type
    Luciferase ; Neurospora Crassa Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Luciferase
  • Alt name
    Luc
  • Species
    Firefly
  • Insert Size (bp)
    1741
  • Mutation
    Codon-optimized for N. crassa

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Pprm-1 Promoter
  • Species
    Neurospora crassa
  • Insert Size (bp)
    1757

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Firefly luciferase codon-optimized for Neurospora crassa was received from Jay Dunlap's Lab (Gooch et al, 2007, Eukaryotic Cell)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pprm1-luciferase; his-3 was a gift from Louise Glass (Addgene plasmid # 137728 ; http://n2t.net/addgene:137728 ; RRID:Addgene_137728)

  • For your References section:

    Integration of Self and Non-self Recognition Modulates Asexual Cell-to-Cell Communication in Neurospora crassa. Fischer MS, Jonkers W, Glass NL. Genetics. 2019 Apr;211(4):1255-1267. doi: 10.1534/genetics.118.301780. Epub 2019 Feb 4. 10.1534/genetics.118.301780 PubMed 30718271
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