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HDAC8 Flag
(Plasmid #13825)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13825 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 +
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDAC8
  • Alt name
    Histone Deacetylase 8
  • Species
    H. sapiens (human)
  • Entrez Gene
    HDAC8 (a.k.a. CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS)
  • Tag / Fusion Protein
    • flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HDAC8 Flag was a gift from Eric Verdin (Addgene plasmid # 13825 ; http://n2t.net/addgene:13825 ; RRID:Addgene_13825)
  • For your References section:

    Histone deacetylase HDAC8 associates with smooth muscle alpha-actin and is essential for smooth muscle cell contractility. Waltregny D, Glenisson W, Tran SL, North BJ, Verdin E, Colige A, Castronovo V. FASEB J. 2005 Jun . 19(8):966-8. 10.1096/fj.04-2303fje PubMed 15772115