|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13825||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 +
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameHistone Deacetylase 8
SpeciesH. sapiens (human)
Entrez GeneHDAC8 (a.k.a. CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS)
/ Fusion Protein
- flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HDAC8 Flag was a gift from Eric Verdin (Addgene plasmid # 13825 ; http://n2t.net/addgene:13825 ; RRID:Addgene_13825)
For your References section:Histone deacetylase HDAC8 associates with smooth muscle alpha-actin and is essential for smooth muscle cell contractility. Waltregny D, Glenisson W, Tran SL, North BJ, Verdin E, Colige A, Castronovo V. FASEB J. 2005 Jun . 19(8):966-8. 10.1096/fj.04-2303fje PubMed 15772115