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pYES2/25Q
(Plasmid #1384)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1384 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYES2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    huntingtin (25Q)
  • Alt name
    htt
  • Alt name
    huntingtin
  • Alt name
    HD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    Exon1 sequences containing the first 17 amino acids followed by 25 glutamines were fused in frame
  • GenBank ID
    AB016794
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GAL1
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

URA3 selection, 2 micron origin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYES2/25Q was a gift from Michael Sherman (Addgene plasmid # 1384 ; http://n2t.net/addgene:1384 ; RRID:Addgene_1384)
  • For your References section:

    Huntington toxicity in yeast model depends on polyglutamine aggregation mediated by a prion-like protein Rnq1. Meriin AB, Zhang X, He X, Newnam GP, Chernoff YO, Sherman MY. J Cell Biol 2002 Jun 10;157(6):997-1004. Epub 2002 Jun 10. 10.1083/jcb.200112104 PubMed 12058016