pIVT-myc-T58A-S62D
(Plasmid #138540)

• Purpose: For making myc-T58A-S62D RNA
• Depositing Lab: Carmen Williams
• Publication: Williams Lab Plasmids 2020 (unpublished)

Backbone

• Vector backbone: pIVT
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 3059
• Total vector size (bp): 4379
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myelocytomatosis oncogene T58A S62D
• Alt name: Myc T58A S62D
• Species: M. musculus (mouse)
• Insert Size (bp): 1320
• Mutation: Threonine 58 changed to alanine and serine 62 changed to aspartic acid
• GenBank ID: NM_001177352.1
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dharmacon

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry