pCKmatBC
(Plasmid #138587)

• Purpose: matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
• Depositing Lab: Markus Jeschek
• Publication: Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.1016/j.ymben.2016.12.013. Epub 2017 Jan 3.

Backbone

• Vector backbone: pCK01
• Total vector size (bp): 6735
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
• Alt name: matBC
• Species: Rhizobium
• Insert Size (bp): 2957
• Mutation: matB contains native GUG start codon
• Promoter: Plac promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CAGGAAACAGCTATGAC
• 3′ sequencing primer: GTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pCKmatBC.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry