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pBS Caronte (CT#88)
(Plasmid #13859)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 13859 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBS SK+
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
  • Entrez Gene
    CER1 (a.k.a. CAR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

full-length caronte

Addgene QC note: Compared to best blast hit for gallus Cer1/Car
(AAD51610.1|AF139721_1), there is one bp mismatch, causing an S2A
residue substitution. The mutation is not known to affect function, and this was likely an accepted isoform when this paper was published in 1999.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS Caronte (CT#88) was a gift from Cliff Tabin (Addgene plasmid # 13859 ; ; RRID:Addgene_13859)
  • For your References section:

    Antagonistic signaling by Caronte, a novel Cerberus-related gene, establishes left-right asymmetric gene expression. Yokouchi Y, Vogan KJ, Pearse RV, Tabin CJ. Cell. 1999 Sep 3. 98(5):573-83. 10.1016/S0092-8674(00)80045-8 PubMed 10490097