pRHA40
(Plasmid #138941)

• Purpose: XOR gate with IPTG and aTc as inputs and mRFP1 as output
• Depositing Lab: Guillermo Rodrigo
• Publication: Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548. doi: 10.1371/journal.pgen.1007548. eCollection 2018 Jul.

Backbone

• Vector backbone: pUC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: riboregulated mRFP1
• Species: Synthetic
• Insert Size (bp): 2189

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: M13fwd
• 3′ sequencing primer: M13rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry