pHD-DsRed-kkv-3::NG
(Plasmid #138960)

• Purpose: Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
• Depositing Lab: Paul Adler
• Publication: Adler et al bioRxiv 2020

Backbone

• Vector backbone: pHD-DsRed
• Backbone size w/o insert (bp): 4048
• Vector type: an editing vector for Drosophila

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 3' end of kkv and neon green
• Alt name: Chitin synthase
• Species: D. melanogaster (fly)
• GenBank ID: NM_079509.3
• Entrez Gene: kkv (a.k.a. Dmel_CG2666, Blimp, CG2666, CS-1, CS-1/kkv, ChSB, DmCS-1, DmeCHS1, DmeChSB, Dmel\CG2666, Kkv, blimp, chitin synthase, l(3)82Fh)
• Tag / Fusion Protein:
  • Neon Green fused to C terminus of Kkv

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAGGCTCAGAAAGAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The vector was obtained from Addgene ((#63166).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/718841v3 for bioRxiv preprint.