PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138960||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4048
Vector typean editing vector for Drosophila
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert name3' end of kkv and neon green
Alt nameChitin synthase
SpeciesD. melanogaster (fly)
Entrez Genekkv (a.k.a. Dmel_CG2666, Blimp, CG2666, CS-1, CS-1/kkv, ChSB, DmCS-1, DmeCHS1, DmeChSB, Dmel\CG2666, Kkv, blimp, chitin synthase, l(3)82Fh)
/ Fusion Protein
- Neon Green fused to C terminus of Kkv
- Cloning method Gibson Cloning
- 5′ sequencing primer AAGGCTCAGAAAGAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/718841v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHD-DsRed-kkv-3::NG was a gift from Paul Adler (Addgene plasmid # 138960 ; http://n2t.net/addgene:138960 ; RRID:Addgene_138960)