pTub-alpha-1-mtGFP
(Plasmid
#138981)
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PurposeNeuron-specific expression of membrane targeted EGFP
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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Alt namemembrane targeted EGFP (mtGFP)
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MutationEGFP (added membrane targeting domain from GAP43).
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTub-alpha-1-mtGFP was a gift from Frank Bradke & Sebastián Dupraz (Addgene plasmid # 138981 ; http://n2t.net/addgene:138981 ; RRID:Addgene_138981) -
For your References section:
Axon Growth of CNS Neurons in Three Dimensions Is Amoeboid and Independent of Adhesions. Santos TE, Schaffran B, Broguiere N, Meyn L, Zenobi-Wong M, Bradke F. Cell Rep. 2020 Jul 21;32(3):107907. doi: 10.1016/j.celrep.2020.107907. 10.1016/j.celrep.2020.107907 PubMed 32698008
