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pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE
(Plasmid #139095)


Item Catalog # Description Quantity Price (USD)
Plasmid 139095 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Staphylococcus pyogenes
  • Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1337R

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE was a gift from Neville Sanjana (Addgene plasmid # 139095 ; ; RRID:Addgene_139095)
  • For your References section:

    High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Legut M, Daniloski Z, Xue X, McKenzie D, Guo X, Wessels HH, Sanjana NE. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. 10.1016/j.celrep.2020.02.010 PubMed 32130891