Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13917||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberLow Copy
Alt namemouse prion protein
SpeciesM. musculus (mouse)
Entrez GenePrnp (a.k.a. AA960666, AI325101, CD230, P, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, S, Sinc, prP27-30, prP33-35C)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Full length mouse PrP with 3F4 epitag was cloned into HindIII and BamHI sites of mammalian cell expression vector pCB6+.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mSecPrP-pCB6+ was a gift from Susan Lindquist (Addgene plasmid # 13917 ; http://n2t.net/addgene:13917 ; RRID:Addgene_13917)
For your References section:Wild-type PrP and a mutant associated with prion disease are subject to retrograde transport and proteasome degradation. Ma J, Lindquist S. Proc Natl Acad Sci U S A 2001 Dec 18;98(26):14955-60. Epub 2001 Dec 11. 10.1073/pnas.011578098 PubMed 11742063
Map uploaded by the depositor.