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RCAS(B) Chfz-1 WT (CT#535)
(Plasmid #13932)

Full plasmid sequence is not available for this item.



This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13932 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Fz 1
  • Alt name
    Frizzled 1
  • Species
    G. gallus (chicken)
  • Entrez Gene
    FZD1 (a.k.a. Fz-1, cFz-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Frizzled 1 wild type ORF: 5’ NcoI-primer, 3’ EcoRI-primer,
shuttle vector: Slax13.

There is a L149V mutation in the gene. There is no data regarding how this mutation affects protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(B) Chfz-1 WT (CT#535) was a gift from Cliff Tabin (Addgene plasmid # 13932 ; ; RRID:Addgene_13932)
  • For your References section:

    Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751