pRC/CMV-TYK2V678F-VSV
(Plasmid #139349)

• Purpose: Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)
• Depositing Lab: Sandra Pellegrini
• Publication: Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9. doi: 10.1074/jbc.M801427200. Epub 2008 May 2.

Backbone

• Vector backbone: pRC/CMV
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TYK2 cDNA with 267nt of 5' UTR
• Species: H. sapiens (human)
• Mutation: V362F-V678F
• Entrez Gene: TYK2 (a.k.a. IMD35, JTK1)
• Promoter: CMV
• Tag / Fusion Protein:
  • VSV (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: BstXI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry