pRC/CMV-TYK2(delta)TK-VSV
(Plasmid
#139353)
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PurposeExpression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRC/CMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTYK2 cDNA expressing aa 1-895
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SpeciesH. sapiens (human)
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MutationV362F-deletion of aa 896-1187
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Entrez GeneTYK2 (a.k.a. IMD35, JTK1)
- Promoter CMV
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Tag
/ Fusion Protein
- VSV (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC/CMV-TYK2(delta)TK-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139353 ; http://n2t.net/addgene:139353 ; RRID:Addgene_139353) -
For your References section:
Distinct domains of the protein tyrosine kinase tyk2 required for binding of interferon-alpha/beta and for signal transduction. Velazquez L, Mogensen KE, Barbieri G, Fellous M, Uze G, Pellegrini S. J Biol Chem. 1995 Feb 17;270(7):3327-34. doi: 10.1074/jbc.270.7.3327. 10.1074/jbc.270.7.3327 PubMed 7531704
