Scarlet I-FRET 10-Venus
(Plasmid
#139366)
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PurposeFRET Standard
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneClontech C1 or N1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScarlet 10AA Venus
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site Mfe1 (unknown if destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer CTC TAC AAA TGT GGT ATG G (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pmScarlet-I-C1 #85044 was used in this construct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Scarlet I-FRET 10-Venus was a gift from Richard Day (Addgene plasmid # 139366 ; http://n2t.net/addgene:139366 ; RRID:Addgene_139366) -
For your References section:
Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells. Day RN, Day KH, Pavalko FM. PLoS One. 2021 Dec 23;16(12):e0261660. doi: 10.1371/journal.pone.0261660. eCollection 2021. 10.1371/journal.pone.0261660 PubMed 34941939
