RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)
(Plasmid #139444)

• Purpose: Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4
• Depositing Lab: Caroline Goujon
• Publication: Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376. doi: 10.1038/s41564-018-0273-9. Epub 2018 Nov 26.

Backbone

• Vector backbone: RRL.sin.cPPT.SFFV/IRES-puro.WPRE
• Backbone manufacturer: Goujon lab, PMID: 30478388
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform)
• Species: H. sapiens (human)
• Insert Size (bp): 682
• Mutation: sequence codon-optimized
• Entrez Gene: NCOA7 (a.k.a. ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3)
• Promoter: SFFV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RRL.sin.cPPT.SFFV/IRES-puro.WPRE backbone modified from backbone described in PMID: 24048477.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry