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pcDNA3-PPO-mScarlet
(Plasmid #139503)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 139503 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5616
  • Total vector size (bp) 7350
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    parapinopsin
  • Alt name
    PPO
  • Species
    Lethenteron camtschaticum
  • Insert Size (bp)
    1029
  • GenBank ID
    AB116380.1
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet following Gly-Gly-Ser linker (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV forward primer
  • 3′ sequencing primer BGH reverse primer
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Akihisa Terakita, Osaka University, Japan
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please see Koyanagi et al., PNAS 2004 (PMID: 15096614) for original cloning of parapinopsin. Please visit https://www.biorxiv.org/content/10.1101/2021.02.19.432008v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-PPO-mScarlet was a gift from Michael Bruchas (Addgene plasmid # 139503 ; http://n2t.net/addgene:139503 ; RRID:Addgene_139503)
  • For your References section:

    A photoswitchable GPCR-based opsin for presynaptic inhibition. Copits BA, Gowrishankar R, O'Neill PR, Li JN, Girven KS, Yoo JJ, Meshik X, Parker KE, Spangler SM, Elerding AJ, Brown BJ, Shirley SE, Ma KKL, Vasquez AM, Stander MC, Kalyanaraman V, Vogt SK, Samineni VK, Patriarchi T, Tian L, Gautam N, Sunahara RK, Gereau RW 4th, Bruchas MR. Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 10.1016/j.neuron.2021.04.026. Epub 2021 May 11. 10.1016/j.neuron.2021.04.026 PubMed 33979635