pcDNA3-PPO-mScarlet
(Plasmid #139503)

• Purpose: Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.
• Depositing Lab: Michael Bruchas
• Publication: Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 10.1016/j.neuron.2021.04.026. Epub 2021 May 11.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5616
• Total vector size (bp): 7350
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: parapinopsin
• Alt name: PPO
• Species: Lethenteron camtschaticum
• Insert Size (bp): 1029
• GenBank ID: AB116380.1
• Promoter: CMV
• Tag / Fusion Protein:
  • mScarlet following Gly-Gly-Ser linker (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV forward primer
• 3′ sequencing primer: BGH reverse primer

Resource Information

• Supplemental Documents:
  • PPO-Scarlet Snapgene file
• A portion of this plasmid was derived from a plasmid made by: Akihisa Terakita, Osaka University, Japan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see Koyanagi et al., PNAS 2004 (PMID: 15096614) for original cloning of parapinopsin. Please visit https://www.biorxiv.org/content/10.1101/2021.02.19.432008v1 for bioRxiv preprint.