pET303C-hSOD1 A4V/C6S
(Plasmid #139667)

• Purpose: Expression plasmids containing human A4V/C6S SOD1
• Depositing Lab: Svetlana Antonyuk
• Publication: Chantadul et al Commun Biol. 2020 Mar 5;3(1):97. doi: 10.1038/s42003-020-0826-3.

Backbone

• Vector backbone: pET303C
• Backbone manufacturer: N/A
• Backbone size w/o insert (bp): 5363
• Total vector size (bp): 5828
• Modifications to backbone: N/A
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Superoxide dismutase-1
• Alt name: SOD1
• Species: H. sapiens (human)
• Insert Size (bp): 465
• Mutation: change alanine 4 to valine, change cysteine 6 to serine
• Entrez Gene: SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pET303C-SOD1 A4V C6S.gb
• A portion of this plasmid was derived from a plasmid made by: GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry