RCAS (A) Hoxd13 (CT#441)
(Plasmid #13985)

• Depositing Lab: Cliff Tabin
• Publication: Goff et al Development. 1997 Feb . 124(3):627-36.

Backbone

• Vector backbone: RCASBP(A)
• Backbone size w/o insert (bp): 11600
• Vector type: Retroviral ; avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Hoxd-13
• Alt name: Hox D13
• Alt name: homeobox D13
• Species: G. gallus (chicken)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: RCAS-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RCASHoxd-13 was constructed using a cDNA corresponding to the longer of the two transcripts whose putative coding region is identical to the published sequence, with the exception of 9 glycines encoded by ggc repeats starting at amino acid position 10, instead of the reported 7 ggc repeats (Rogina and Upholdt, 1993). This cDNA was cloned into the shuttle vector SLAX-13 (Riddle et al., 1993), and then subcloned into the ClaI site of RCAS(BP)A .