RCAS(A) mdnTcf4Ben (CT#1130)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14020||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; avian expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
SpeciesM. musculus (mouse)
MutationDominant negative Tcf4B, engrailed fusion
Entrez GeneTcf7l2 (a.k.a. TCF4B, TCF4E, Tcf-4, Tcf4)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2–50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3′end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS(A) mdnTcf4Ben (CT#1130) was a gift from Cliff Tabin (Addgene plasmid # 14020 ; http://n2t.net/addgene:14020 ; RRID:Addgene_14020)
For your References section:A Tcf4-positive mesodermal population provides a prepattern for vertebrate limb muscle patterning. Kardon G, Harfe BD, Tabin CJ. Dev Cell. 2003 Dec . 5(6):937-44. 10.1016/S1534-5807(03)00360-5 PubMed 14667415