|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140554||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4495
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameThe 2nd generation DA sensor GRAB_DA2h (high affinity)
SpeciesH. sapiens (human)
Insert Size (bp)1857
- Promoter hSyn
- Cloning method Gibson Cloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-hsyn-GRAB_DA2h was a gift from Yulong Li (Addgene plasmid # 140554 ; http://n2t.net/addgene:140554 ; RRID:Addgene_140554)
For your References section:Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun F, Zhou J, Dai B, Qian T, Zeng J, Li X, Zhuo Y, Zhang Y, Wang Y, Qian C, Tan K, Feng J, Dong H, Lin D, Cui G, Li Y.. Nature Methods (2020) 10.1038/s41592-020-00981-9