pZDonor rosa26 LSL-mMALAT1
(Plasmid
#140691)
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Purposegenerate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region
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Depositing Lab
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Depositing OrganizationCleveland Clinic Foundation
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140691 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 140691-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 140691-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepZDonor
- Total vector size (bp) 14753
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Modifications to backboneadd Loxp Stop Loxp and mouse MALAT1
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemouse MALAT1
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Alt nameNR_002847.3
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SpeciesM. musculus (mouse)
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Insert Size (bp)6983
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GenBank IDNR_002847.3 NR_002847.3
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Entrez GeneMalat1 (a.k.a. 2210401K01Rik, 9430072K23Rik, Neat2)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 140691-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 140691-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZDonor rosa26 LSL-mMALAT1 was a gift from Jianjun Zhao (Addgene plasmid # 140691 ; http://n2t.net/addgene:140691 ; RRID:Addgene_140691)