Reciever_plasmid2
(Plasmid
#140695)
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PurposeEncodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMB1
- Backbone size w/o insert (bp) 3231
- Total vector size (bp) 3950
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Modifications to backboneROP element reduces copy number
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfCFP
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SpeciesSynthetic
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Insert Size (bp)719
- Promoter engineered rhl promoter (responds to C4-HSL)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tatggatgcggcgggac
- 3′ sequencing primer gccagtgtgagacagcggtgcggac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Reciever_plasmid2 was a gift from Matthew Bennett (Addgene plasmid # 140695 ; http://n2t.net/addgene:140695 ; RRID:Addgene_140695) -
For your References section:
Spatiotemporal Dynamics of Synthetic Microbial Consortia in Microfluidic Devices. Alnahhas RN, Winkle JJ, Hirning AJ, Karamched B, Ott W, Josic K, Bennett MR. ACS Synth Biol. 2019 Sep 20;8(9):2051-2058. doi: 10.1021/acssynbio.9b00146. Epub 2019 Aug 9. 10.1021/acssynbio.9b00146 PubMed 31361464
Map uploaded by the depositor.
