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(Plasmid #140753)


Item Catalog # Description Quantity Price (USD)
Plasmid 140753 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 7498
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    Changed Histidine 92 to Alanine
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dead-Lapronic/pcDNA3.1(-) was a gift from Luis Felipe Barros (Addgene plasmid # 140753 ; ; RRID:Addgene_140753)
  • For your References section:

    Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded FRET Indicator. Galaz A, Cortes-Molina F, Arce-Molina R, Romero-Gomez I, Mardones GA, Barros LF, San Martin A. Anal Chem. 2020 Jun 29. doi: 10.1021/acs.analchem.0c01741. 10.1021/acs.analchem.0c01741 PubMed 32600029