PurposeTargeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140760||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2396
- Total vector size (bp) 18841
Vector typeMouse Targeting
Growth in Bacteria
Insert Size (bp)1032
- Promoter Endogenous Sox1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Sox1(2A-Cre) was a gift from Hideyuki Okano (Addgene plasmid # 140760 ; http://n2t.net/addgene:140760 ; RRID:Addgene_140760)
For your References section:Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H. Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938. 10.1242/dev.174938 PubMed 31597657