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phlFs-mLin
(Plasmid #140792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140792 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 140792-D50 50 μg of DNA in Tris buffer $495
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DNA 140792-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    FRT-Hygromycin
  • Total vector size (bp) 7520
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phlFs-mLinearizer
  • Alt name
    CMV-phlFO-PhlFR-2A-mCherry
  • Insert Size (bp)
    3468
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATCAAGTGTATCATATGCC
  • 3′ sequencing primer TGTTTGTCCACCACCGGTAGAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phlFs-mLin was a gift from Gabor Balazsi (Addgene plasmid # 140792 ; http://n2t.net/addgene:140792 ; RRID:Addgene_140792)

  • For your References section:

    Multiplexed Gene Expression Tuning with Orthogonal Synthetic Gene Circuits. Szenk M, Yim T, Balazsi G. ACS Synth Biol. 2020 Apr 17;9(4):930-939. doi: 10.1021/acssynbio.9b00534. Epub 2020 Mar 23. 10.1021/acssynbio.9b00534 PubMed 32167761
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