Oct4deltaPE-53BP1mCherry
(Plasmid #141122)

• Purpose: Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells
• Depositing Lab: John Schimenti
• Publication: Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.1002/dvg.23368. Epub 2020 Apr 28.

Backbone

• Vector backbone: mouse deltaPE Oct4-GFP transgenic reporter (addgene plasmid #52382)
• Total vector size (bp): 22128
• Modifications to backbone: removed eGFP and added a PacI restriction site
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fragment of human 53BP1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: FseI (not destroyed)
• 5′ sequencing primer: ttaattaaCCACCATGGTGAGCAAG
• 3′ sequencing primer: ggccggccCTCATTCACCGGTGTTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #52382 (with the modifications described) was used as the backbone. The insert sequence was cloned from addgene plasmid #19835

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.01.29.925487v1 for bioRxiv preprint.