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GA-MFF
(Plasmid #141160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141160 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 141160-D50 50 μg of DNA in Tris buffer $495
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DNA 141160-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pAcGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4652
  • Total vector size (bp) 5681
  • Modifications to backbone
    GA dimertization dependent fluorescent protein was cloned in using NheI /BspEI to replace AcGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitochondrial fission factor
  • Alt name
    MFF, isoform 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1029
  • GenBank ID
    NM_020194 NM_020194
  • Entrez Gene
    MFF (a.k.a. C2orf33, EMPF2, GL004)
  • Tag / Fusion Protein
    • GA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer pFastBac Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GA-MFF was a gift from Gia Voeltz (Addgene plasmid # 141160 ; http://n2t.net/addgene:141160 ; RRID:Addgene_141160)

  • For your References section:

    Fission and fusion machineries converge at ER contact sites to regulate mitochondrial morphology. Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK. J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122. 10.1083/jcb.201911122 PubMed 32328629
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