PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||141174||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameC6 HaloTyr tRNA synthetase
MutationL270S, Y271L, N311G, C313T
- Promoter CMV
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAcBac1-haloTyrRS C6 was a gift from Ryan Mehl (Addgene plasmid # 141174 ; http://n2t.net/addgene:141174 ; RRID:Addgene_141174)